cspC [2019-09-04 09:21:38]

cold shock protein

locus: BSU_05120

pI: 4.52

mw: 7.12 kDa

protein length: 66 aa

gene length: 201 bp

function: RNA chaperone

product: cold shock protein

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.2|RNA chaperones]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.5|Cold stress proteins]

Gene

Coordinates: 559,264 → 559,464

The protein

Paralogous protein(s)

• [protein|0F89D0B03F54B283CDD99A940D43C18E6B5703BB|CspD], [protein|16E048FE5AF76CC771DEBBA8CB56B75A5414B3A2|CspB]

Structure

• [PDB|2I5M] ([protein|16E048FE5AF76CC771DEBBA8CB56B75A5414B3A2|CspB], 71% identity) [pubmed|17481655]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|D453E1E7D4256B56AFB2149731E08B104D59431B|cspC]
  description: [pubmed|22383849]

Biological materials

Mutant

• GP1969 (Δ[gene|D453E1E7D4256B56AFB2149731E08B104D59431B|cspC]::spec trpC2) available in [SW|Jörg Stülke]'s lab
• BKE05120 (Δ[gene|D453E1E7D4256B56AFB2149731E08B104D59431B|cspC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE05120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTTGTTGCCTCCTAGT, downstream forward: _UP4_TAATCTTCAATCGTTTATAC
• BKK05120 (Δ[gene|D453E1E7D4256B56AFB2149731E08B104D59431B|cspC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK05120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTTGTTGCCTCCTAGT, downstream forward: _UP4_TAATCTTCAATCGTTTATAC

Expression vector

• Constitutive overexpression of cspC with pGP3124 (in [SW|pBQ200]), available in [SW|Jörg Stülke]'s lab

lacZ fusion

• GP1984 ([gene|DE4857E8A228F145EFB9B7AD817585258344ED1F|amyE]::(P_cspC-lacZ cat) trpC2) available in [SW|Jörg Stülke]'s lab

two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in [SW|Stülke] lab

References

• 12399512,12171653,11752341,11717297,12838604,9379903,9914312,11591689,9920884,12427936,17481655